The Ad4BP/SF1 Function of Testes BACAd4BPtTAZ Transgenic-Knockout Mice normally regulated during Development.*)

Fatchiyah1,2,3, Mohamad Zubair2, Ken-Ichirou Morohashi2,3

1 Lab. of Molecular & Cellular Biology, Department of Biology, Brawijaya University, Malang 65145, Indonesia, 2Division of Sex Differentiation, National Institute for Basic Biology, National Institutes of Natural Sciences, Okazaki-Japan 444-8787, 3Department of Molecular Biomechanics, School of Life Science, The Graduate University for Advanced Studies (SOKENDAI), Okazaki 444-8585, Japan.


To address the function of Ad4BP/SF1 in developing gonad on Ad4BP/SF1 knockout mouse, we have modified the BACAd4BP endogenous by homologous recombinant with modification cassette-containing Tet-Off system and LacZ reporter genes to allow the expression of Ad4BP/SF1 in developing tissues and organ differentiation, regulate of Ad4BP/SF1 mechanism, and reveal the defect due to Ad4BP/SF1 deficiency. The BACAd4BPtTAZ recombinant has a potential to express a lacZ reporter gene and Ad4BP/SF-1 in the tissues where the endogenous Ad4BP/SF-1 gene is expressed. Expectedly, the lacZ expressions in the BAC transgenic mice mostly recapitulated the endogenous gene expressions. However, protein level of upregulated Ad4BP/SF-1 varied among the transgenic mice. Showing a good correlation with the expression levels, the transgene differentially affected the target tissues. We further applied the BAC-transgenic mice to rescue Ad4BP/SF-1 gene disrupted mouse. Interestingly, although the Ad4BP/SF1 protein expression levels are slightly high in testes of BACd4BPtTAZ-Tg mice, the BAC recombinants successfully rescued and normally developed the gonad of the KO mouse.

Keywords: Ad4BP, SF1, BAC transgenic, Steroidogenesis, Testis

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