Revealing Spleen Ad4BP/SF1 Knockout Mouse by BAC-Ad4BP-tTAZ Transgene

Book Series IFMBE Proceedings
ISSN 1680-0737 (Print) 1433-9277 (Online)
Volume Volume 23
Book 13th International Conference on Biomedical Engineering
Publisher Springer Berlin Heidelberg
DOI 10.1007/978-3-540-92841-6
Copyright 2009
ISBN 978-3-540-92840-9 (Print) 978-3-540-92841-6 (Online)
Part Track 5
DOI 10.1007/978-3-540-92841-6_477
Pages 1920-1923
Subject Collection Engineering
SpringerLink Date Sunday, March 15, 2009

Fatchiyah Contact Information, M. Zubair4 and K. I. Morohashi5

(3) Dept. of Biology, School of Science, Brawijaya University, Veteran, Malang, 65145, Indonesia
(4) Dept. of Internal Medicine Endocrinology, UT South Western Medical Centre, Dallas, Texas, USA
(5) Dept. of Molecular Biology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan

The purpose of this study is addressed the function of Ad4BP/SF1 in developing spleen of Ad4BP/SF-1 KO mouse. I have modified the BAC-Ad4BP endogenous by homologous recombinant with modification cassette-containing tetracycline transactivator system and LacZ reporter genes to allow the expression of Ad4BP/SF1 in developing tissues and organ differentiation, regulate of Ad4BP/SF1 mechanism, and reveal the defect due to Ad4BP/SF1 deficiency. Recently studies, by immunihistochemistry of adult tissues showed that revealed coincident expressing between Ad4BP/SF-1 and lacZ, indicating the tTA system seemed to reproduces the endogenous expression of Ad4BP/SF-1, and thus likely to lacZ, Ad4BP/SF-1 in the BAC transgene was expected to be expressed in the corresponding tissues under the control of the bidirectional promoter. Interestingly, the BAC-Ad4BP-tTA transgenic mice were succeeded to up regulate the expression of Ad4BP/SF-1 at the protein level than wild type. And the fetal spleen of Tg(+);Ad4(+/+) was larger than that of the wild type, and expectedly the size of the spleen of Tg(+);Ad4(-/-) apparently recovered.

Keywords  Ad4BP/SF-1 – knockout mouse – Spleen – BAC transgenic – homologous recombinant

IFMBE Proceedings 13th International Conference on Biomedical Engineering ICBME 2008 3–6 December 2008 Singapore 10.1007/978-3-540-92841-6_477 Chwee Teck Lim and James C. H. Goh

download for details