Pertemuan ke

Sub pokok bahasan

Metoda pembelajaran

1 Overview molecular biology techniques. Lab. biosafety, basic  and sterile techniques, pippetting  test Lecture F
2 Basic analysis of DNA and RNA Lecture F
3 DNA Electrophoresis: Agorose & Polyacrilamide Lecture & practice F
4 Amplification DNA by PCR, RT-PCR and qPCR Lecture & practice F
5 DNA Cloning Techniques: Vector, DNA target, manipulation enzyme Lecture & practice ELA
6 DNA Reccombinant Transformation, microbe cell culture and single clone selection Lecture & practice ELA
7 Single clone of DNA Recombinant identification by PCR or RFLP Lecture & practice ELA
8 Middle test
9 Gene Expression DNA Recombinant: Basic Analysis of Protein Lecture SWD
10 Protein isolation and purifcation from clone Lecture & practice SWD
11 SDS PAGE Lecture & practice SWD
12 Immunoblotting: dot-blot and Western blot Lecture & practice W
12 Immnunohistochemistry Lecture W
14 S C L Report presentation TIM
15 S C L Report presentation TIM

MK TAGM untuk Pascasarjana S2 Biologi adalah MK implementasi IGN-TTRC UB Council Module 1 untuk Biologi Molekuler.

Tim Dosen:

Fatchiyah, M.Kes., PhD. (F, Koordinator), Dr.Ir.Estri Laras Arumingtyas, MScSt (ELA), Dr. Sri Widyarti, M.Si (SWD) dan Widodo, MS, Ph.D (W)

Acuan Bacaan

Arif IA, Bakir MA, Khan HA, Al Farhan AH, Al Homaidan AA, Bahkali AH, Sadoon MA, Shobrak M. A brief review of molecular techniques to assess plant diversity. Int J Mol Sci. 2010 May 10;11(5):2079-96.

Fatchiyah. Arumingtyas EL, Widyarti S, Rahayu S. Biologi Molekuler: Prinsip Dasar Analisis: Penerbit Erlangga, Jakarta. 2011.

Fierro AC, Vandenbussche F, Engelen K, Van de Peer Y, Marchal K.  Meta Analysis of Gene Expression Data within and Across Species. Curr Genomics. 2008 Dec;9(8):525-34.

Kremer A, Caron H, Cavers S, Colpaert N, Gheysen G, Gribel R, Lemes M, Lowe AJ, Margis R, Navarro C, Salgueiro F. Monitoring genetic diversity in tropical trees with multilocus dominant markers. Heredity. 2005 Oct;95(4):274-80.

Alves RR, Alves HN . The faunal drugstore: animal-based remedies used in traditional medicines in Latin America . J Ethnobiol Ethnomed. 2011 Mar 7;7:9.

Thomas TR, Kavlekar DP, LokaBharathi PA.Marine drugs from sponge-microbe association–a review.Mar Drugs. 2010 Apr 22;8(4):1417-68.

Finkeldey R, Leinemann L, Gailing O. Molecular genetic tools to infer the origin of forest plants and wood. Appl Microbiol Biotechnol. 2010 Feb;85(5):1251-8. Epub 2009 Nov 13.

Nellen, W. 2011. IGN-TTRC Biodiversity Molecular Biology module 1: training of trainers & student course. Implementation Indonesian German Network for Teaching, Training and Research Collaborations (IGN-TTRC) UB Council. Kassel University. German


WebLink: Genbank, SwissProt, etc



This July, the IGN-TTRC lecture will be take by Prof Michael Blake (who one famous professor of DNA Barcoding in the World)  and DR.Stefan Smith from Munich University German

Let’s send Your Application as soon as possible!

If you need the application form –>please send email to or, or take a soft copy on LSIH UB lt 2


Implementation IGN for TTR Biodiversity Curricula

Training of Trainers and Students Course of Module II on DNA Barcoding

Brawijaya University

Malang, July 1-14, 2012

At the Second Year activity, The Indonesian – German Network for Teaching, Training and Research Collaborations (IGN-TTRC), a consortium to improve teaching, training and research collaborations within Indonesian universities and between those of Indonesia and Germany, will give a competitive, merit-based scholarship program that provides opportunities for two weeks: one week Training of Trainers (Lecturers) and a one week Students Course in Module II on DNA Barcoding, conducted at the University of Brawijaya. The ToT and Student Course consist of lectures and a practical class given by German and Indonesian tutors. Candidates from the following Universities are eligible participants for the program and involve UB and UNAND (main course Universities), UNSRAT, UNEJ, IPB, UNMUL, and UNAIR (associated Universities). This program is supported by the German Academic Exchange Service (Deutscher Akademischer Austausch Dienst, DAAD), by the Ministry of National Education of the Republic Indonesia, and several other funding sources.

The biological diversity of each country is a valuable and vulnerable natural resource. Sampling, identifying, and studying biological specimens are among the first steps towards protecting and benefiting from this biodiversity. DNA Barcoding is new tool for taxonomic research based on sequence diversity in short, standardized genomic regions. The international DNA Barcoding initiative is generating a global, open access library of reference barcode sequences that will allow the reliable identification of biological specimens by non-taxonomists. In principle, DNA Barcoding requires three consecutive steps: a. obtaining and preparing organisms (collecting, identification, preparation, and preserving), b. Laboratory procedure: sampling and processing specimen samples to obtain DNA barcode gene sequences, and c. data management using a dedicated database.

Program Objectives

  1. To give an overview of the role of molecular biology methods in biodiversity research. The course modules will be integrated into the biodiversity curricula at the participating universities. The final goal would be a joint the M.Sc and/or PhD curriculum.
  2. To establish a curriculum that is equivalent to a German MSc degree and that enables Indonesian students to rapidly integrate into a German PhD program. Credits will be awarded by the IGN-TTRC. The participating universities may accept these certificates as partial fulfillment of an Indonesian PhD degree.

Eligibility Requirements

–       Student Applicants must have Grade Transcripts of (≥3.25) proven by a certified original or certified photocopy of academic records (transcripts) from any previous university work

–       Applicant must have active English language proficiency and a TOEFL/TOEIC/IELTS score of min. 450/550/5.5

–       Applicants should provide a motivation letter of 2-3 pages (including CV) describing their scientific interest.

–       Lecturer Applicants must fill in and sign a Commitment Letter of Module Implementation

–       Applicants, except UB participants, should include a statement by the University/Faculty if costs for travel and subsistence can be (partially) covered if the available funding is not sufficient.

–       Applicant should fill in IGN-TTRC application form and send it together with the other material by E-Mail to along.


Selection Process of Module II on DNA Barcoding

Selection of students and graduate students

All participating institutions will motivate their best students to apply for the courses. Candidates will write a letter of motivation accompanied by a brief evaluation by their mentor. The council will decide on acceptance based on excellence of the candidates. In the case of equal qualification, we will try to accommodate students from different partner universities. It is understood that gender issues will be strictly observed.

Funding for travel and subsistence is limited to approx. 10 persons. Up to 20 (max.) students/lecturers may be accepted for each course if additional funding is provided by the faculty/university.

Selection will be made by the IGN-TTRC Council academic review committee that includes UNKA, UB, UNAND, IPB, UNSRAT, UNMUL, UNEJ and UNAIR.  In selecting eligible candidates, the review committee will consider academic merit as well as evidence of leadership potential and experience capacity for change; adaptability; ability to pursue independent study; English language proficiency; creativity; initiative; self-expression.

Application Deadline
·         A complete application must be received by IGN-TTRC Council of the University of Brawijaya, Fatchiyah, PhD: no later than April 20th 2012. Please write the subject email ToT UB Module 2 for lecturers or SC UB Module 2 for students.


Award Provisions and Conditions

Scholarship recipients will receive the following:

  • Free tuition fee for TOT and student course
  • Credits Certificate will be awarded by the IGN-TTRC
  • For participants from outside UB (UNSRAT, UNEJ, IPB, UNMUL, and UNAIR) will be reimbursed for transportation from their university to UB


Award Decision, Start Dates and Venue

Award decisions are expected to be announced on Website and/or email to inviting participant by June 1, 2012.

Courses take place on July 1-14, 2012 (UB Malang)

UB Venue: at Central Lab of Life Sciences UB (LSIH UB)


Written and E-mail Inquiries

Email inquiries may be addressed to