MK Rekayasa Protein (Protein Engineering)

lecture material:

Lecture 1 III. recombinant protein

Lecture 2 Protein Complex and Protein-protein Interaction


Protein engineering is the process of developing useful or valuable proteins. It is a young discipline, with much research taking place into the understanding of protein folding and recognition for protein design principles.

There are two general strategies for protein engineering, ‘rational’ protein design and directed evolution. These techniques are not mutually exclusive; researchers will often apply both. In the future, more detailed knowledge of protein structure and function, as well as advancements in high-throughput technology, may greatly expand the capabilities of protein engineering. Eventually, even unnatural amino acids may be incorporated thanks to a new method that allows the inclusion of novel amino acids in the genetic code.

PROTEIN ENGINEERING Principles and Practice was edited by JEFFREY L. CLELAND CHARLES S. CRAIK wrote that:

Proteins are involved in every aspect of life-structure, motion, catalysis, recognition and regulation. Protein Engineering: Principles and Practice provides a basic framework for understanding both proteins and protein engineering. This comprehensive book covers general, yet essential knowledge required for successful protein engineering, including everything from the fundamentals to modifying existing proteins and developing new proteins.

The book begins by introducing the main concepts of protein engineering, including: understanding protein conformation, comprehending the relationship between protein composition and structure, and potential methods for predicting a protein’s conformation.