Jadual kuliah Rekayasa protein (S2 biologi)

                                            Protein Engineering

 (S2 BIOLOGY)

SEMESTER GANJIL TAHUN 2013-2014

16 September 2013 – 22 Januari 2014

Lecturers      :  Prof. Fatchiyah, Ph.D (F)

Widodo, Msi, PhD (W)

Dr. Sri Widyarti (SW)

Sofy Permana, D.Sc (SP)

Day             :  Thursday

Time            :  07.30-09.20

Venue          :  MP 2.6

No Date Topics

Competencies

Lecturer

1

19 Sep’13

Introduction

  1. Bond and Energy in Protein makeup
  2. Amino Acid and their character
  3. Protein Engineering scop
  4. Application of protein engineering
Understand about protein and application of protein engineering

W

2

26 Sep’13

Protein Structure, function and classification

  1. The structure and classification of protein
  2. Protein synthesis and modification
  3. Protein degradation and traficking
  4. DNA-binding protein, membrane protein, immunoglobulin, transport protein, chaperone and enzyme
Understand about  protein structure and classification of protein

W

3

03 Okt’13

Protein Folding, missfolding and aggregation

  1. Protein folding mechanism
  2. Factor significant for protein folding process and stability
  3. Protein behavior in missfolding and aggregation
Understand about Protein Folding, missfolding and aggregation

 

SW

4

10 Okt’13

 

Protein purification, identification and structure analysis

  1. Protein Isolation system
  2. Protein Purification system
  3. Technique to identify protein
Understand about techniques of  Protein purification, identification and structure analysis

 

SW

5

17 Okt’13

Enzyme catalysis and kinetics Understand about Enzyme catalysis and kinetics

SW

6

24 Okt’13

7

31 Okt’13

Technique to analize protein structure Understand abaout the technique to analize structure of protein

W

8

7 Nov’13

Peptide Engineering and nanotechnology

 

Understand about current technology of Peptide Engineering and nanotechnology

W

9

14 Nov’13

Protein expression system

  1. Vector for protein expression system
  2. Promoter for protein expression
  3. Protein taging
  4. Cells host for protein expression

 

Understand to develope Protein expression system

 

F

10

21 Nov’13

11

28 Nov’13

Methods of protein modification

  1. PCR-based mutagenesis
  2. De novo protein design
Understand about methods for protein modification

F

12

5 Des’13

Ethic and Economics prospect Understand about  ethic and economics prospect of protein engineering

F

13

12 Des’13

Case study of Protein Engineering:

  1. Designing hybrid protein of MAP-2
  2. Confirmation properties dan activity of hybrid protein
  3. Properties of protein in vitro and in vivo
Understand about application of protein engineering/hybrid protein, examination of protein properties

SP

14

19 Des’13

SP

15

26 Des’13

 

SP

16

2 Jan’14

Design a simple research plan for a biotechnology invention

 

(Student presentation)

Proficient to  develope research proposal on new idea to solve human probelm by using protein engineering technology.

SP

 

 

 

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