Revealing Spleen Ad4BP/SF1 Knockout Mouse by BAC-Ad4BP-tTAZ Transgene
| Book Series | IFMBE Proceedings |
| ISSN | 1680-0737 (Print) 1433-9277 (Online) |
| Volume | Volume 23 |
| Book | 13th International Conference on Biomedical Engineering |
| Publisher | Springer Berlin Heidelberg |
| DOI | 10.1007/978-3-540-92841-6 |
| Copyright | 2009 |
| ISBN | 978-3-540-92840-9 (Print) 978-3-540-92841-6 (Online) |
| Part | Track 5 |
| DOI | 10.1007/978-3-540-92841-6_477 |
| Pages | 1920-1923 |
| Subject Collection | Engineering |
| SpringerLink Date | Sunday, March 15, 2009 |
Fatchiyah 3 
, M. Zubair4 and K. I. Morohashi5
| (3) | Dept. of Biology, School of Science, Brawijaya University, Veteran, Malang, 65145, Indonesia |
| (4) | Dept. of Internal Medicine Endocrinology, UT South Western Medical Centre, Dallas, Texas, USA |
| (5) | Dept. of Molecular Biology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan |
Abstract
The purpose of this study is addressed the function of Ad4BP/SF1 in developing spleen of Ad4BP/SF-1 KO mouse. I have modified the BAC-Ad4BP endogenous by homologous recombinant with modification cassette-containing tetracycline transactivator system and LacZ reporter genes to allow the expression of Ad4BP/SF1 in developing tissues and organ differentiation, regulate of Ad4BP/SF1 mechanism, and reveal the defect due to Ad4BP/SF1 deficiency. Recently studies, by immunihistochemistry of adult tissues showed that revealed coincident expressing between Ad4BP/SF-1 and lacZ, indicating the tTA system seemed to reproduces the endogenous expression of Ad4BP/SF-1, and thus likely to lacZ, Ad4BP/SF-1 in the BAC transgene was expected to be expressed in the corresponding tissues under the control of the bidirectional promoter. Interestingly, the BAC-Ad4BP-tTA transgenic mice were succeeded to up regulate the expression of Ad4BP/SF-1 at the protein level than wild type. And the fetal spleen of Tg(+);Ad4(+/+) was larger than that of the wild type, and expectedly the size of the spleen of Tg(+);Ad4(-/-) apparently recovered.
Keywords Ad4BP/SF-1 – knockout mouse – Spleen – BAC transgenic – homologous recombinant
IFMBE Proceedings 13th International Conference on Biomedical Engineering ICBME 2008 3–6 December 2008 Singapore 10.1007/978-3-540-92841-6_477 Chwee Teck Lim and James C. H. Goh
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