JADUAL KULIAH MK TEKNIK ANALISIS GENETIKA MOLEKULER
PASCASARJANA BIOLOGI FMIPA MALANG
| Pertemuan ke |
Sub pokok bahasan |
Metoda pembelajaran |
Dosen |
| 1 | Overview molecular biology techniques. Lab. biosafety, basic and sterile techniques, pippetting test | Lecture | F |
| 2 | Basic analysis of DNA and RNA | Lecture | F |
| 3 | DNA Electrophoresis: Agorose & Polyacrilamide | Lecture & practice | F |
| 4 | Amplification DNA by PCR, RT-PCR and qPCR | Lecture & practice | F |
| 5 | DNA Cloning Techniques: Vector, DNA target, manipulation enzyme | Lecture & practice | ELA |
| 6 | DNA Reccombinant Transformation, microbe cell culture and single clone selection | Lecture & practice | ELA |
| 7 | Single clone of DNA Recombinant identification by PCR or RFLP | Lecture & practice | ELA |
| 8 | Middle test | ||
| 9 | Gene Expression DNA Recombinant: Basic Analysis of Protein | Lecture | SWD |
| 10 | Protein isolation and purifcation from clone | Lecture & practice | SWD |
| 11 | SDS PAGE | Lecture & practice | SWD |
| 12 | Immunoblotting: dot-blot and Western blot | Lecture & practice | W |
| 12 | Immnunohistochemistry | Lecture | W |
| 14 | S C L | Report presentation | TIM |
| 15 | S C L | Report presentation | TIM |
MK TAGM untuk Pascasarjana S2 Biologi adalah MK implementasi IGN-TTRC UB Council Module 1 untuk Biologi Molekuler.
Tim Dosen:
Fatchiyah, M.Kes., PhD. (F, Koordinator), Dr.Ir.Estri Laras Arumingtyas, MScSt (ELA), Dr. Sri Widyarti, M.Si (SWD) dan Widodo, MS, Ph.D (W)
Acuan Bacaan
Arif IA, Bakir MA, Khan HA, Al Farhan AH, Al Homaidan AA, Bahkali AH, Sadoon MA, Shobrak M. A brief review of molecular techniques to assess plant diversity. Int J Mol Sci. 2010 May 10;11(5):2079-96.
Fatchiyah. Arumingtyas EL, Widyarti S, Rahayu S. Biologi Molekuler: Prinsip Dasar Analisis: Penerbit Erlangga, Jakarta. 2011.
Fierro AC, Vandenbussche F, Engelen K, Van de Peer Y, Marchal K. Meta Analysis of Gene Expression Data within and Across Species. Curr Genomics. 2008 Dec;9(8):525-34.
Kremer A, Caron H, Cavers S, Colpaert N, Gheysen G, Gribel R, Lemes M, Lowe AJ, Margis R, Navarro C, Salgueiro F. Monitoring genetic diversity in tropical trees with multilocus dominant markers. Heredity. 2005 Oct;95(4):274-80.
Alves RR, Alves HN . The faunal drugstore: animal-based remedies used in traditional medicines in Latin America . J Ethnobiol Ethnomed. 2011 Mar 7;7:9.
Thomas TR, Kavlekar DP, LokaBharathi PA.Marine drugs from sponge-microbe association–a review.Mar Drugs. 2010 Apr 22;8(4):1417-68.
Finkeldey R, Leinemann L, Gailing O. Molecular genetic tools to infer the origin of forest plants and wood. Appl Microbiol Biotechnol. 2010 Feb;85(5):1251-8. Epub 2009 Nov 13.
Nellen, W. 2011. IGN-TTRC Biodiversity Molecular Biology module 1: training of trainers & student course. Implementation Indonesian German Network for Teaching, Training and Research Collaborations (IGN-TTRC) UB Council. Kassel University. German
WebLink: Genbank, SwissProt, etc