Feb

20

Hasil-hasil Riset peneliti RG Smonagenes UB disitasi dalam Sigma-Aldrich bisa dicek di

Pogestemon herba

Pogestemon herba

http://www.sigmaaldrich.com/catalog/papers/25484897

http://www.sigmaaldrich.com/catalog/papers/23559751#

Pachouil alcohol

Pachouil alcohol

http://www.sigmaaldrich.com/catalog/papers/61adea67-e075-46a4-8c40-00af77f419ab#

http://www.sigmaaldrich.com/catalog/papers/26483598

http://www.sigmaaldrich.com/catalog/papers/26005274#

http://www.sigmaaldrich.com/catalog/papers/8e007c84-23a7-4a3a-a358-407eaa2f7552#

http://www.sigmaaldrich.com/catalog/papers/aabdb08b-4ab0-41ec-ade7-1b91555c4e93

http://www.sigmaaldrich.com/catalog/papers/3997fd80-a96d-487f-a74d-db48b9ac8c3f

http://www.sigmaaldrich.com/catalog/papers/90d936e5-aeb0-4343-8a1f-2d70b712692a

 

Obese Rambutan peel

Obese Rambutan peel

PPAR gamma CSN1S2

PPAR gamma rambutan peel

CSN1S2 RA disease RAGE

CSN1S2 RA disease RAGE

CSN1S2 Pre-Osteoblast

CSN1S2 Pre-Osteoblast

CSN1S2 Diabetes

CSN1S2 Diabetes

CSN1S2 RA disease

CSN1S2 RA disease

CSN1S2 Jak-Stat3

CSN1S2 Jak-Stat3

Feb

20

Bioethics research:

  • Morality is a unique feature of the life of human beings. It is deeply influenced by several cultural factors, such as history, traditions, education, religious beliefs, etc.
  • The intellectual analysis of this human dimension in all of its complexity is the goal of the discipline called Ethics. Ethics does not create morality or moral behavior.
  • The goal of ethics is much more modest: to explore the nature of moral experience, its universality and its diversity.
  • Ethics and morality are generally taken as synonyms, because they originally had the same meaning: the study of the disposition, character, or attitude of a specific person, group of people or culture, and ways of promoting or perfecting it.

As a field of study, psychology examines a broad range of research and applied areas. Important parts of such work are teaching and research on the behavior of nonhuman animals, which contribute to the understanding of basic principles underlying behavior and to advancing the welfare of both human and nonhuman animals. While psychologists must conduct their teaching and research in a manner consonant with relevant laws and regulations, ethical concerns further mandate that psychologists consider the costs and benefits of procedures involving animals before proceeding with these activities.

The 3Rs Principle of Replacement, Reduction and Refinement is central to the Guidelines. Since the concept of the 3Rs was first introduced by William Russell and Rex Burch in The Principles of Humane Experimental Technique in 1959, the 3Rs have been internationally accepted as the basis of the care and use of animals for scientific purposes. These are: to Replace the need for animal use by alternative means, to Reduce
the numbers of animals used to an unavoidable minimum, and to Refine any procedures necessarily used, so as to minimize the impact on animals, consistent with the achievement of a justifiable scientific purpose, and which is necessary because there is no other way of achieving that purpose. The incorporation of the 3Rs at the planning stages ensures that full consideration of the principle is exercised at every juncture of the process.

1. care-animal-non human research guidelines (PDF)

2. NACLAR-guide Lines using animal model (PDF)

bioethic syllabi WHO

Lect 2 Laik Ethik riset dengan Hewan Coba FAT

Lec 1 – Bioethics-Research

JADWAL MK Bioetika penelitian hayati

 

 

Feb

20

Hari                 : Rabu

Pukul               : 09.20 – 11.05 WIB

Tempat            : MC3.1

 

NO.

TANGGAL

TOPIK

DOSEN

1.

18-2-2015 Pendahuluan

F

2.

25-2-2015 Dasar-dasar browsing and Searching GeneBank: Nucleotide Database F

3.

4-3-2015 Nucleotide sequence analysis F

4.

11-3-2015 dbSNP sequence Variation (Kuis) F

5.

18-3-2015 Student Presentation: Nucleotide database to genome mapping and analysis (SCL) F

6.

25-3-2015 Polymorfisme and variation genome

F

7.

1-4-2015 Comparative genome, Taxonomy and evolution (Kuis)

F

8.

8-4-2015 Student Presentation: Polymorphisme to taxonomy mapping (SCL)

F

9.

15-4-2015

UJIAN TENGAH SEMESTER

F

 

10.

29-4-2015 Dasar-dasar browsing and Searching GeneBank: protein and PDB Database

W

11.

6-5-2015 Protein sequence analysis

W

12.

13-5-2015 3D protein analysis (kuis)

W

13.

20-5-2015 Protein folding

W

14.

27-5-2015 Protein network pathway (TT)

W

15.

3-6-2015 Student Presentation: 3D protein to protein folding & network pathway (SCL)

W

UJIAN AKHIR SEMESTER

W

Keterangan: F : Prof. Fatchiyah, M.Kes., Ph.D.  W         : Widodo, S.Si., M.Si., Ph.D.Med.Sc.

 

SCL:  berupa mini projek.

 

ASSESMENT

Nilai Kuliah  (NK):

  • Ujian Tengah Semester          : 35%
  • Ujian akhir                               : 35%
  • Kuis                                         :10%
  • Diskusi/keaktifan di kelas       :10%
  • Tugas                                      : 10%

 

Nilai Praktikum (NP):

  • Placements test                      : 20 %
  • Laporan                                   : 40 %
  • Ujian Akhir Praktikum             : 40 %

 

Nilai Akhir  (NA) :   (2 NK + 1 NP)/3

Skor Nilai Akhir (Nilai Huruf = NH):

≥80            : A

76-<80       : B+

70- <76      : B

60- <70      : C+

56- <60      : C

50- <56      : D+

46- <50      : D

>46            : E

 

Daftar Pustaka:

  1. Fatchiyah. 2015. Prinsip Dasar Bioinformatika. UB Press. Malang
  2. Marketa Zvelebil and Jeremy O. Baum, 2008, Understanding bioinformatic, Garland Science, Taylor and Francis group publisher
  3. Jean-Michel Claverie, Ph. D., Cedric Notredame, Ph.D. 2006, Bioinformatics For Dummies, 2nd Edition, For Dummies Publisher
  4. Cynthia Gibas and Per Jambeck, 2001, Developing Bioinformatics Computer Skills, O’Reilly Media publisher.
  5. Huaiyu Mi and Paul Thomas. Methods in Molecular Biology, 2009, Volume 563, Part 2, 123-140
  1. GeneBank: NCBI GeneBank: www.ncbi.nlm.nih.gov/,
  2. DDBJ: http://www.ddbj.nig.ac.jp/
  3. Embl: http://www.ebi.ac.uk/
  4. Protein analysis: http://www.expasy.ch/sprot/sprot-top.html
  5. protein characterization http://www.mips.biochem.mpg.de dan http://www.protomap.cs.huji.ac.il
  6. Database alligment sequence: Hovergen http://pbil.univ-lyon1.fr/databases/hovergen.html (vertebrate alignments)
  7. Pfam http://www.sanger.ac.uk/Software/Pfam/ (protein domain alignments and profile HMMs)
  8. BLOCKS http://blocks.fhcrc.org/

14. Ribosomal Database Project http://rdp.cme.msu.edu/html/ alignments and trees derived from rRNA sequences

Feb

17

Hari                 : Kamis

Pukul               :09.20 – 11.05 WIB

Tempat            :R. Biologi

NO.

TANGGAL

TOPIK

DOSEN

1.

26-2-2015

Pendahuluan

ELA

2.

5-3-2015

Teknik dasar analisis bahan genetic (DNA, RNA) dan cara pengukuran biomolekul secara kualitatif & kuantitatif

ELA

3.

12-3-2015

Dasar teknik amplifikasi DNA-RNA dan aplikasinya (SCL)

ELA

4.

19-3-2015

Pembuatan Probe

F

5.

26-3-2015

Analisis polimorfisme & Manipulasi genetik

F

6.

2-4-2015

Hibridisasi: Sistem deteksi gen & level mRNA (kuiz)

F

7.

9-4-2015

DNA sequencing  (SCL)

F

8.

16-4-2015

UJIAN TENGAH SEMESTER

TIM

9.

23-4-2015

 

10.

30-4-2015

Dasar teknik-teknik kloning Molekuler (kuiz) F

11.

7-5-2015

Teknik dasar isolasi dan presipitasi protein SW

12.

21-5-2015

Pembuatan kurva protein standard dan mengukur kadar  Protein (SCL) SW

13.

28-5-2015

Electrophoresis protein

SW

14.

4-6-2015

Analisis Protein (immunoblotting)

SW

15.

11-6-2015

Immunohistochemistry (SCL)

SW

16.

UJIAN AKHIR SEMESTER

TIM

Keterangan: F : Prof. Fatchiyah, M.Kes., Ph.D., ELA: Prof. Dr. Ir. Estri Laras Arumningtyas, M.Sc.St., SW: Dr. Sri Widyarti, M.Si.

 

SCL: diisi dengan mini projek dari kelompok mahasiswa yang telah ditetapkan.

 

ASSESMENT

Nilai Kuliah  (NK):

  • Ujian Tengah Semester          : 35%
  • Ujian akhir                               : 35%
  • Kuis                                         :10%
  • Diskusi/keaktifan di kelas       :10%
  • Tugas                                      : 10%

 

Nilai Praktikum (NP):

  • Placements test                      : 20 %
  • Laporan                                   : 40 %
  • Ujian Akhir Praktikum             : 40 %

 

Nilai Akhir  (NA) :   (NK + NP)/2

Skor Nilai Akhir (Nilai Huruf = NH):

≥80            : A

76-<80       : B+

70- <76      : B

60- <70      : C+

56- <60      : C

50- <56      : D+

46- <50      : D

>46            : E

 

D. DAFTAR PUSTAKA

  1. Fatchiyah, Sri Widyarti, Estri Laras Arumingtyas, Sri Rahayu, 2008, Teknik Dasar Analisis Biologi Molekuler, Universitas Brawijaya, Malang.
  2. Fatchiyah, Sri Widyarti, Estri Laras Arumingtyas, Sri Rahayu, 2011. Biologi Molekuler: Prinsip Dasar Analisis. Penerbit Erlangga, Jakarta.
  3. Andrews AT. 1980. Electrophoresis: Theory, Techniques & Biochemical and Clinical Apllication. 2nd Ed. Clarendon Press, Oxford.
  4. Ausubel FM., Brent R., Kingston RE., Moore D., Seidman JG. Smith JA. Struhl K. 2002. Short Protocols in Molecular Biology. 5rd Ed. John Wiley & Sons.
  5. GeneBank: NCBI GeneBank: www.ncbi.nlm.nih.gov/,5

DDBJ: http://www.ddbj.nig.ac.jp/

Embl: http://www.ebi.ac.uk/

  1. Innis MA. Gelfand DH., Sninsky JJ. 1999. PCR Application Protocol for Functional Genomics. Academics Press
  2. Sambrook J. & Russel DW. 2001. Molecular Cloning: A laboratory manual. Cold Spring Harbor.  www.cshl.org/sambrook
  3. Bollag DM., & Edelstein SJ. 1991. Protein Methods. A John Wiley & Sons.
  4. Harlow E. & Lane D. 1988. Antibodies: A laboratory manual. ColdSpring
  5. Harbor Konfermann R. & Dubel S. 2001. Antibody Engeneering. Springer Lab. Manual. www.duebel.uni-hd.de
  6. Robyt JF & White BJ. 1990. Biochemical Techniques: Theory & Practice. Brooks/Cole Pub.
  7. Wilson K & Walker J. 2004. Principles & Techniques of Practical Biochemistry. 4th Ed. Cambridge University Press. www.cup.cam.ac.uk/wilson  Atau www.cup.org/wilson

Feb

8

My group publication has been published on Research-gate
https://www.researchgate.net/profile/Fatchiyah_Fatchiyah/publications/

2015

Fatchiyah F, Setiawan B,Suharjono S, Noor Z. The anti-osteoporosis effects of CSN1S2 protein of goat milk and yoghurt on a complete Freund’s adjuvant-induced rheumatoid arthritis model in rats. Biomarkers and Genomic Medicine (2015b) xx, 1-8. http://dx.doi.org/10.1016/j.bgm.2015.10.001

Vita Agustina, Bambang Setiawan , Fatchiyah Fatchiyah. 2015. Acute toxicity of caprine alpha S2-casein protein on the microstructures and mineral profiles of rat ileum. Asian Pacific Journal of Tropical Disease, 08/2015; 5(9):721-725. DOI:10.1016/S2222-1808(15)60920-3

B Tong, G Q Gao, Y Muramatsu, T Ohta, H Kose, G P Li, F Fatchiyah, T Yamada. Association of the expression levels in the longissimus muscle and a SNP in the CDC10 gene with marbling in Japanese Black beef cattle. Meat Science 108:28-31 May 2015

Rista Nikmatu Rohmah, Ferlany Hardiyanti, Fatchiyah Fatchiyah. Inhibition on JAK-STAT3 Signaling Transduction Cascade Is Taken by Bioactive Peptide Alpha-S2 Casein Protein from Goat Ethawah Breed Milk. Acta Informatica Medica 07/2015; 23(4):233-238. DOI:10.5455/aim.2015.23.233-238