material lecture:
1. DNA fingerprinting and barcoding (fat-UB 2015)
2. Lecture Intro Forensic Science DNA Testing
3. 5.dbSnp of nucleotide Seq Variation
Sep
14
material lecture:
1. DNA fingerprinting and barcoding (fat-UB 2015)
2. Lecture Intro Forensic Science DNA Testing
3. 5.dbSnp of nucleotide Seq Variation
Sep
6
Matriks Rencana Pembelajaran Semester Mata Kuliah Sidik Jari Molekuler.
Minggu ke |
Kemampuan Akhir yang Diharapkan (CLO) |
Bahan kajian |
Pokok Bahasan (topics) |
Sub Pokok Bahasan (subtopics) |
Bentuk Pembelajaran (Learning methods) |
Indikator/Kriteria Penilaian (Value Criteria) |
Bobot Nilai (%) |
Fasilitas Pembelajaran (learning Facility) |
1 | Menjelaskan Dasar-dasar sidik jari DNA dan DNA typing dalam forensik | Dasar-dasar sidik jari DNA dan DNA typing dalam forensik | Overview Dasar-dasar sidik jari DNA dan DNA typing dalam forensik | DNA Fingerprinting & DNA typing on Forensic Biology | Lecture and discussion | Kebenaran dan ketepatan analisis |
6 |
Laptop, LCD, module |
2 | Melakukan pengambilan sampel, purifikasi, penyimpanan | Pengambilan sampel, purifikasi, penyimpanan | Sample tracking | Sample collection, extraction, purification & storage | Presentation, Lecture and discussion | Kebenaran dan ketepatan analisis |
6 |
Laptop, LCD, module, paper exam |
3 | Menetapkan metode isolasi dan analysis sampel molecular dan sekuensing | Molecular sample preparation | isolasi dan analysis sampel molecular dan sekuensing | DNA isolation, PCR, DDGE and Sequencing | Presentation, Lecture and discussion | Kebenaran dan ketepatan analisis |
10 |
Laptop, LCD, module, paper exam |
4 | Memahami perbedaan genome dan mitokodria typing | Genetic basis of DNA typing | Basic Analysis of DNA typing | Genomic or mitokondria DN A | Presentation, Mini Project Lecture and discussion | Kebenaran dan ketepatan analisis |
6 |
Laptop, LCD, module, paper exam |
5 | Membedakan Fingerprinting DNA & Forensic DNA system | Comparation of Fingerprinting DNA & Forensic DNA system | Fingerprinting DNA & Forensic DNA system | CODIS, Y-chromosome, SNP, STR, SSP, HLA typing | Presentation, Mini Project Lecture and discussion | Kebenaran dan ketepatan analisis |
6 |
Laptop, LCD, module, paper exam |
6 | Menganlisis data molekuler secara in silico | Analisis data molekuler secara in silico | Bioinformatic Analysis | DNA Seq analysis, alignment, phylogenetic tree | Presentation, mini project, Lecture and discussion | Kebenaran dan ketepatan analisis |
10 |
Laptop, LCD, module, paper exam |
7 | Menjelasakan Dasar teori DNA Barcoding | Dasar teori DNA Barcoding | DNA Barcoding analysis | What is DNA Barcoding? Biomarker for DNA animal barcode | Presentation, Lecture and discussion | Kebenaran dan ketepatan analisis |
6 |
Laptop, LCD, module, paper exam |
8 | Middle test |
|
||||||
9 | Membandingkan analisis DNA Barcoding pada biodiversitas dan kekerabatan hewan | Analisis DNA Barcoding pada biodiversitas dan kekerabatan hewan | DNA Barcoding analysis on animal molecular biodiversity and taxonomy | Barcode of Life Data Systems (BOLD) database
GeneBank Database for DNA & Protein |
Presentation, Mini Project Lecture and discussion | Kebenaran dan ketepatan analisis |
16 |
Laptop, LCD, module, paper exam |
10 | Membandingkan dasar analisis kekerabatan spesies | Dasar-dasar analisis kekerabatan spesies | Molecular data Interpretation | UPGM, statistics analysis, Clad | Presentation, Lecture and discussion | Kebenaran dan ketepatan analisis |
6 |
Laptop, LCD, module, paper exam |
11 | Membandingkan analisis DNA Barcoding pada biodiversitas dan kekerabatan pada mikrobia | Analisis DNA Barcoding pada biodiversitas dan kekerabatan pada mikrobia | Biomarker for microbe DNA Barcoding analysis | 16SRNA, SNP, SST | Presentation, Mini Project Lecture and discussion | Kebenaran dan ketepatan analisis |
6 |
Laptop, LCD, module, paper exam |
12 | Membandingkan dasar analisis DNA Barcoding utuk kekerabatan spesies | Dasar analisis DNA Barcoding utuk kekerabatan spesies | DNA Barcoding analysis on molecular biodiversity and taxonomy of microbe | Bacteri, Virus, Jamur | Presentation, Lecture and discussion | Kebenaran dan ketepatan analisis |
6 |
Laptop, LCD, module, paper exam |
13-14 | Menenetapkan biomarker untuk analisis sisdik jari pada tanaman | Type biomarker untuk analisis sisdik jari pada tanaman | Biomarker for plant DNA Fingertyping and Barcoding analysis | Minisatelite, microsatelite | Presentation, Lecture and discussion | Kebenaran dan ketepatan analisis |
6 |
Laptop, LCD, module, paper exam |
15 | Membandingkan analisis DNA Barcoding pada biodiversitas dan kekerabatan pada tanaman | Analisis DNA Barcoding pada biodiversitas dan kekerabatan pada tanaman | DNA Fingerprinting analayis on molecular biodiversity and taxonomy of plant | RFLP, Simple sequence length polymorphisms (SSLP), RAPD | Presentation, Mini Project, Lecture and discussion | Kebenaran dan ketepatan analisis |
6 |
Laptop, LCD, module, paper exam |
Daftar Pustaka
John M Butler. 2009. Fundamentals of Forensic DNA Typing 1st Edition. Academic Press. ISBN-13: 978-0123749994. ISBN-10: 0123749999
Richads Li. 2015. Forensic Biology. 2nd Edition. CRS. · ISBN-10: 1439889708 · ISBN-13: 978-1439889701
Jörg Epplen and Thomas Lubjuhnn. 1999. DNA Profiling and DNA Fingerprinting. Birkhäuser. ISBN-10: 3764360186. ISBN-13: 978-3764360184
Ricki Lewis. 2011. Human Genetics concepts and application. McGraw-Hill Education; 10 edition ISBN-13: 978-0073525303, ISBN-10: 0073525308 or ISBN: 007246268x
Tom Strachan & Andrew Read.2003. Human Molecular genetics. Garland Science; 3 edition. · ISBN-10: 0815341822 · ISBN-13: 978-0815341826
Julian Knight.2009. Human Genetic Diversity. Oxford Univrsity Press. London.
Robert Nussbaum, Roderick R. McInnes, and Huntington F Willard. 2007. Genetics in Medicine, 7th Edition. Sounders. · ISBN: 9781416030805
Ida Lopez and David L. Erickson. 2012. DNA Barcodes: Methods and Protocols (Methods in Molecular Biology). Human Press. ISBN-13: 978-1617795909. ISBN-10: 1617795909
Nikolaus J. Sucher and James R. Hennell. 2012. Plant DNA Fingerprinting and Barcoding: Methods and Protocols (Methods in Molecular Biology). Human Press. ISBN-13: 978-1617796081. ISBN-10: 1617796085
Erich Grotewold and Joseph Chappel. 2015. Plant Genes, Genomes and Genetics. Willey Blackwell. ISBN-13: 978-1119998877. ISBN-10: 1119998875
Cecie Starr, Ralph Taggart, Christine Evers, & Lisa Starr. Biology: The Unity and Diversity of Life 14th Edition. Brooks Cole. ISBN-13: 978-1305073951. ISBN-10: 1305073959
Gene Helfman and Bruce B. Collette. 2009. The Diversity of Fishes: Biology, Evolution, and Ecology. Wiley-Blackwell; 2 edition. ISBN-10: 1405124946. ISBN-13: 978-1405124942
Jacques Izard and Maria Rivera. 2014. Metagenomics for Microbiology. !rst Ed. Academic Press. ISBN-13: 978-0124104723. ISBN-10: 012410472X
Sandra Tscherwizek. 2008. 16S Ribosomal RNA Gene Sequencing: Establishment of a Method for the Identification of Microorganisms in Biopharmaceutical Production Areas. VDM Verlag Dr Muller. ISBN-13: 978-3639109030. ISBN-10: 3639109031
Sep
17
JADUAL KULIAH MK TEKNIK ANALISIS DNA FORENSIK DAN BARCODING
PASCASARJANA BIOLOGI FMIPA MALANG
Pertemuan ke |
Sub pokok bahasan |
Metoda pembelajaran |
Dosen |
1 | Overview DNA Fingerprinting & DNA typing on Forensic Biology | Lecture | F |
2 | Genetic basis of DNA typing (genomic or mtDNA) | Lecture | F |
3 | Forensic DNA typing system (CODIS, Y-chromosome, SNP, STR, SSP, DDGE) | discussion of articles on forensic DNA testing | F |
4 | Sample collection. extraction, purification, & storage | Practice | F/NK |
5 | DNA preparation and Sequensing | Practice | F/NK |
6 | Bioinformatic Analysis: DNA Seq analysis, alignment, phylogenetic tree | Practice, online | F/NK |
7 | Molecular data Interpretation (UPGM, stat analysis, Clad) | Practice, online | F/NK |
8 | Middle test | ||
9 | Biomarker for animal DNA Barcoding analysis | Lecture | SH |
10 | DNA Barcoding analysis on animal molecular biodiversity and taxonomy | discussion of articles on insect DNA Barcoding | SH |
11 | DNA Barcoding analysis on animal molecular biodiversity and taxonomy | discussion of articles on insect DNA Barcoding | SH |
12 | Biomarker for microbe DNA Barcoding analysis | Lecture | NK |
12 | DNA Barcoding analysis on molecular biodiversity and taxonomy of microbe | discussion of articles on microbe DNA typing & Barcoding | NK |
14 | Biomarker for plant DNA Fingertyping and Barcoding analysis | Lecture | ELA |
15 | DNA Fingerprinting analayis on molecular biodiversity and taxonomy of plant | discussion of articles on Plant DNA Fingerprinting | ELA |
16 | DNA Fingerprinting analayis on molecular biodiversity and taxonomy of plant | discussion of articles on Plant DNA Fingerprinting | ELA |
Tim Dosen:
Fatchiyah, M.Kes., PhD. (F, Koordinator), Dr.Ir.Estri Laras Arumingtyas, MScSt (ELA), Dr.Suharjono, MS (SH), dan Nia Kurniawan, MP, Ph.D. (NK)
Acuan Bacaan
Bashinski, J. “Laboratory Standards: Accreditation, Training, and Certification of Staff in the Forensic Context.” p. 159-173. Ballantyne J, Sensabaugh G. DNA Technology and Forensic Science, Cold Spring Harbor, NY: Cold Spring Harbor Press, 1989.
Balke, M. and Schmidt S. 2012. Training of Trainers and Students Module II: DNA Barcoding Course material. Implementation of Indonesian-German Network for Teaching, Training and Research Collaborations (IGN-TTRC) UB Councils. Zoologische Staatssammlung, Munchen. German
Hughey, J. Bio 13- Introduction To Forensic DNA Analysis Lecture Manual: Section 5052, Fall 2011.
Kobilinsky, L., Liotti, T. F. & Oeser-Sweat, J. DNA: Forensic and Legal Applications. Wiley-Interscience, New Jersey, 2004. ISBN: 0471414786.
Kirby, L.T. 1990. DNA Fingerprinting: An Introduction. W. H. Freeman.
Krawczak, M. & J. Schmidtke. 1994. DNA Fingerprinting. Books International, Inc.
Moriniere, Jerome. 2012. Apractical lab manual for molecularphylogenetics, barcoding and friends. Bavarian state collection of zoology. Zoologische Staatssammlung, Munchen. German
OSHA Bloodborne Pathogen Standard. 1992. Fisher Scientific.
Saferstein, R. 2001. Forensic Science Handbook. Prentice Hall
Weedn VW, Roby RK. Forensic DNA Testing. Arch Pathol Lab Med 1993;117:486-491
Weblink:
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0014448
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0028832
Feb
21
This July, the IGN-TTRC lecture will be take by Prof Michael Blake (who one famous professor of DNA Barcoding in the World) and DR.Stefan Smith from Munich University German
Let’s send Your Application as soon as possible!
If you need the application form –>please send email to ignttrc.ub.council@gmail.com or praba.sanjaya@gmail.com, or take a soft copy on LSIH UB lt 2
Implementation IGN for TTR Biodiversity Curricula
Training of Trainers and Students Course of Module II on DNA Barcoding
Brawijaya University
Malang, July 1-14, 2012
At the Second Year activity, The Indonesian – German Network for Teaching, Training and Research Collaborations (IGN-TTRC), a consortium to improve teaching, training and research collaborations within Indonesian universities and between those of Indonesia and Germany, will give a competitive, merit-based scholarship program that provides opportunities for two weeks: one week Training of Trainers (Lecturers) and a one week Students Course in Module II on DNA Barcoding, conducted at the University of Brawijaya. The ToT and Student Course consist of lectures and a practical class given by German and Indonesian tutors. Candidates from the following Universities are eligible participants for the program and involve UB and UNAND (main course Universities), UNSRAT, UNEJ, IPB, UNMUL, and UNAIR (associated Universities). This program is supported by the German Academic Exchange Service (Deutscher Akademischer Austausch Dienst, DAAD), by the Ministry of National Education of the Republic Indonesia, and several other funding sources.
The biological diversity of each country is a valuable and vulnerable natural resource. Sampling, identifying, and studying biological specimens are among the first steps towards protecting and benefiting from this biodiversity. DNA Barcoding is new tool for taxonomic research based on sequence diversity in short, standardized genomic regions. The international DNA Barcoding initiative is generating a global, open access library of reference barcode sequences that will allow the reliable identification of biological specimens by non-taxonomists. In principle, DNA Barcoding requires three consecutive steps: a. obtaining and preparing organisms (collecting, identification, preparation, and preserving), b. Laboratory procedure: sampling and processing specimen samples to obtain DNA barcode gene sequences, and c. data management using a dedicated database.
– Student Applicants must have Grade Transcripts of (≥3.25) proven by a certified original or certified photocopy of academic records (transcripts) from any previous university work
– Applicant must have active English language proficiency and a TOEFL/TOEIC/IELTS score of min. 450/550/5.5
– Applicants should provide a motivation letter of 2-3 pages (including CV) describing their scientific interest.
– Lecturer Applicants must fill in and sign a Commitment Letter of Module Implementation
– Applicants, except UB participants, should include a statement by the University/Faculty if costs for travel and subsistence can be (partially) covered if the available funding is not sufficient.
– Applicant should fill in IGN-TTRC application form and send it together with the other material by E-Mail to ignttrc.ub.council@gmail.com along.
Selection of students and graduate students
All participating institutions will motivate their best students to apply for the courses. Candidates will write a letter of motivation accompanied by a brief evaluation by their mentor. The council will decide on acceptance based on excellence of the candidates. In the case of equal qualification, we will try to accommodate students from different partner universities. It is understood that gender issues will be strictly observed.
Funding for travel and subsistence is limited to approx. 10 persons. Up to 20 (max.) students/lecturers may be accepted for each course if additional funding is provided by the faculty/university.
Selection will be made by the IGN-TTRC Council academic review committee that includes UNKA, UB, UNAND, IPB, UNSRAT, UNMUL, UNEJ and UNAIR. In selecting eligible candidates, the review committee will consider academic merit as well as evidence of leadership potential and experience capacity for change; adaptability; ability to pursue independent study; English language proficiency; creativity; initiative; self-expression.
Award Provisions and Conditions
Scholarship recipients will receive the following:
Award decisions are expected to be announced on Website http://lsih.ub.ac.id and/or email to inviting participant by June 1, 2012.
Courses take place on July 1-14, 2012 (UB Malang)
UB Venue: at Central Lab of Life Sciences UB (LSIH UB)
Email inquiries may be addressed to ignttrc.ub.council@gmail.com
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